Production of human growth hormone (hGH) by recombinant DNA technologyHuman growth hormone is recombiannt protein hormone produced in the pituitary gland at the base of the brain mediating both growth and stature. It is currently used in the treatment of "hypopituitary dwarfism," a children's disease in which the pituitary malfunctions and secretes insufficient amounts of hGH for normal growth, and results in dwarfism. Hypopituitary dwarfism is currently synthesis of human growth hormone by recombinant dna technology by administering hGH extracted from the pituitaries of human cadavers. Deca mos 138 evo this is a rare disease, hGH supplies are now so limited that many suffering from hypopituitary dwarfism are unable to receive treatment. The successful bacterial production of hGH will enable the production of sufficient quantities of hGH to treat all those hypopituitary patients who could benefit from its administration. In addition to being efficacious for dwarfism, hGH may have value in treating diffuse gastric bleeding, burns, bone dnz, bone fractures, and other diseases. Supplies of hGH have been so limited tecnology research has been restricted in these areas, reclmbinant now adequate supplies of hGH produced by microbial sources will enable clinicians to investigate these important therapeutic uses.
RECOMBINANT INSULIN AND GROWTH HORMONE PRODUCTION |authorSTREAM
Automatically changes to Flash or non-Flash embed. WordPress Embed Customize Embed. Insulin is of three types: Miles stones passed in insulin production In , Canadian scientists Frederick G.
Banting and Charles H. Best successfully purified insulin from a dog's pancreas. Later Insulin was extracted from the pancreas of cattle and pigs and purified which produce immune reactions in some patients In , the Eli Lilly Corporation produced a human insulin that became the first approved genetically engineered pharmaceutical product.
Materials used for insulin: Materials used for insulin Human insulin is grown in the lab inside common bacteria. Escherichia coli Human protein that produces insulin containing about 20 amino acids Large fermentation tanks to grow the bacteria Nutrients needed for the bacteria Centrifuge, various chromatography and x-ray crystallography instruments.
Structure of Insulin The insulin gene is a protein consisting of two separate chains of amino acids, an A above a B chain, that are held together with bonds. The insulin A chain consists of 21 amino acids and the B chain has Before becoming an active insulin protein, insulin is first produced as preproinsulin. After preproinsulin , the chain evolves into proinsulin , still a single chain but without the signaling sequence. Active protein insulin, the protein without the section linking the A and B chains.
Types of Insulin Production: Types of Insulin Production Type-I Here two insulin chains are grown separately and inserted into plasmids and grown in E. Then 2 chains are joined by oxidation-reduction reaction using lysosomes and cyanogen bromide Then purified by chromatography and other techniques.
Type-II it is also known as Proinsulin process The sequence that codes for proinsulin is inserted into the non-pathogenic E.
Which clumps less and disperses more readily into the blood. Strategy for insulin production PowerPoint Presentation: Human Insulin Production by Bacteria 6 join the plasmid and human fragment and cut with a restriction enzyme PowerPoint Presentation: This makes it possible to create a wide variety of different gene fragments.
Restriction enzyme animation PowerPoint Presentation: Human Insulin Production by Bacteria Mix the recombinant plasmid with bacteria. Screening bacterial cells to learn which contain the human insulin gene is the hard part. This is the step when gene cloning takes place. The single recombinant plasmid replicates within a cell. Then the single cell with many recombinant plasmids produces trillions of like cells with recombinant plasmid — and the human insulin gene. One cell with the recombinant plasmid PowerPoint Presentation: Route to the Production by Bacteria of Human Insulin The final steps are to collect the bacteria, break open the cells, and purify the insulin protein expressed from the recombinant human insulin gene.
Insulin crystals from the purification process: Insulin crystals from the purification process PowerPoint Presentation: These processes together are responsible for longitudinal growth,production in muscle and bones ,break down of fats etc Inadequate production of GH results in short stature, defined as a below normal height for a given age Traditional treatment of Dwarfism: Traditional treatment of Dwarfism Children were treated with injection of GH from deceased humans.
No other animals GH were used in contrast to Insulin. Atleast 8 cadavers were needed to treat a child of age 1 which is continued for years There is also chance of transmission of diseases Growth Hormone: Structure of growth hormone It composed of amino acid residue linked by disulfide bridges in 2 peptide loops 2 hGH genes are seen in human 1. Strategy for production of growth hormone PowerPoint Presentation: Upload from Desktop Single File Upload.
The presentation is successfully added In Your Favorites. Miles stones passed in insulin production: Human Insulin Production by Bacteria 6 join the plasmid and human fragment and cut with a restriction enzyme.
One cell with the recombinant plasmid. Insulin crystals from the purification process. Human growth hormone hGH, or Somatotropin: These processes together are responsible for longitudinal growth,production in muscle and bones ,break down of fats etc Inadequate production of GH results in short stature, defined as a below normal height for a given age.
Traditional treatment of Dwarfism: Atleast 8 cadavers were needed to treat a child of age 1 which is continued for years There is also chance of transmission of diseases.
Structure of growth hormone: Production of recombinant GH: You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.
Go to Application Have a question?